thymosin beta-4 peptide · studied for wound and tissue repair, cell migration, heart and eye repair (thymosin beta-4 trials), flexibility and injury recovery (user reports)

TB-500 dosage

TB-500 is the research name for thymosin beta-4 or its active fragment. Full-length thymosin beta-4 has been through phase 1 and 2 human trials, by IV and as a topical gel, and was tolerated at IV doses up to 1,260 mg a day for 14 days. The injectable TB-500 sold as a research peptide has no human trials of its own. Users commonly load 2 to 2.5 mg twice a week for 4 to 6 weeks, then drop to 1 to 2 mg once a week.

By the Peptide Calculations editors · Updated 2026-10-19

2 mg to 2.5 mgper dose, 2 to 3 times weekly (loading)

Basis: commonly reported. loading phase, 4 to 6 weeks

How often: twice weekly while loading, then once weekly
When: any time; many pair it with the same days as BPC-157
How long: 4 to 6 weeks loading, 4 to 8 weeks maintenance, about 8 to 12 weeks total, then a break
Route: subcutaneous, abdomen or near the injury; intramuscular is also reported
Storage: lyophilized: fridge or freezer; reconstituted: fridge, commonly used within 4 weeks (commonly reported)

Dose ranges by source

Range	Per	Basis	Note
2 mg to 2.5 mg	dose, 2 to 3 times weekly (loading)	commonly reported	loading phase, 4 to 6 weeks
0.75 mg to 2 mg	dose, 1 to 2 times weekly (maintenance)	commonly reported	maintenance, 4 to 8 weeks
42 mg to 1260 mg	day, intravenous, 14 days	clinical trial (phase 1, Ruff et al., 2010, full-length thymosin beta-4)	40 healthy volunteers, no dose-limiting toxicity; this is a different molecule and route from research TB-500

Titration schedule

Step up on this schedule; stay a step longer if side effects are rough.

Weeks	Dose
1-4	2 to 2.5 mg twice weekly
5-6	2 mg twice weekly
7-12	1 to 2 mg once weekly

TB-500 dose to units chart

10 mg vial + 2 mL bacteriostatic water = 5 mg/mL (50 mcg per unit on a U-100 insulin syringe). 10 mg + 2 mL = 5 mg/mL, so 2.5 mg = 0.5 mL = 50 units; 2 mg = 40 units, 1 mg = 20 units. A 5 mg vial with 1 mL gives the same 5 mg/mL.

Dose	mL	Units (U-100)	Doses per vial
2 mg	0.4	40	5
2.25 mg	0.45	45	4
2.5 mg	0.5	50	4

Different vial or water? Full 10 mg + 2 mL table or set your own numbers below.

Commonly stacked with

BPC-157 250 mcg to 500 mcg

Side effects

injection site redness
head rush or lightheadedness right after injecting
fatigue or lethargy
headache
temporary flushing

Before you start. Human dosing is not established for the research peptide. The human trials used full-length thymosin beta-4 by IV or as a gel, not the subcutaneous fragment people buy, so those safety data do not transfer. Because it drives cell migration and blood vessel growth, anyone with cancer or a cancer history should avoid it. It is banned in sport. Not medical advice; start low and talk to a clinician.

TB-500 dosage questions

How much TB-500 should I take?

There is no established human dose. The commonly reported pattern is a loading phase of 2 to 2.5 mg twice a week for 4 to 6 weeks, then 1 to 2 mg once a week. On a 10 mg vial in 2 mL, 2.5 mg is 50 units.

How many units is 2.5 mg of TB-500?

With 10 mg in 2 mL (5 mg/mL), 2.5 mg is 0.5 mL, which is 50 units on a U-100 syringe. With 5 mg in 2 mL it would be 100 units, a full syringe. Larger doses are why many people reconstitute TB-500 more concentrated than BPC-157.

TB-500 vs BPC-157, which is better for injury?

Neither has a human trial for sports injury. BPC-157 has far more animal data for tendon and ligament, and TB-500's parent molecule has human trials for skin wounds and heart repair. Most users run both, which is the Wolverine stack.

Is TB-500 the same as thymosin beta-4?

Not exactly. Thymosin beta-4 is the full 43 amino acid protein; TB-500 vials may contain the full protein or just the 7 amino acid active fragment (LKKTETQ), and labels are not always clear. The human trial doses above were full-length thymosin beta-4 given by IV.

Sources

Brands we recommend for TB-500. We are independent and have no affiliation with any brand: no affiliate links, no referral codes, no paid placements. These are the suppliers whose testing and certificates hold up when we check them.

Licensed Peptides two accredited labs on every lot, certificates public, card and Apple Pay checkout
Core Peptides long track record, public certificates, wide catalog
Ascension Peptides public third-party testing, clear pricing

This page is an educational summary of published labels, trials and commonly reported use. It is not medical advice and does not replace a clinician.