NAD+ dosage
NAD+ is a coenzyme, not a peptide, sold in 100 to 1,000 mg vials. Clinics run it IV at 250 to 1,000 mg over several hours; the one published human infusion study used 750 mg over 6 hours. Subcutaneous use is 50 to 100 mg per shot, one to three times a week, sometimes daily. Inject slowly. Fast delivery causes flushing, chest tightness and nausea.
Basis: clinical trial (Grant et al. 2019, Frontiers in Aging Neuroscience, 8 men, pharmacokinetic pilot). about 2 mg per minute; the authors chose it because it matched a common clinic regimen; no adverse events
- How often
- subcutaneous: 1 to 3 times a week, up to daily; IV: 1 to 5 sessions a week in a course
- When
- morning or midday; it can be stimulating
- How long
- clinic courses run 4 to 6 weeks; subcutaneous use is often ongoing at 1 to 2 shots a week
- Route
- subcutaneous (abdomen) or IV in a clinic; inject subcutaneous doses slowly over a minute or more
- Storage
- lyophilized: fridge, protect from light; reconstituted: fridge, use within 2 to 4 weeks (commonly reported); it degrades faster than peptides
Dose ranges by source
| Range | Per | Basis | Note |
|---|---|---|---|
| 750 mg over 6 hours IV | infusion | clinical trial (Grant et al. 2019, Frontiers in Aging Neuroscience, 8 men, pharmacokinetic pilot) | about 2 mg per minute; the authors chose it because it matched a common clinic regimen; no adverse events |
| 250 mg to 1,000 mg IV | infusion | commonly reported | clinic IV drips over 2 to 4 hours, 1 to 5 times a week in short courses |
| 50 mg to 100 mg subcutaneous | injection | commonly reported | 1 to 3 times a week, some daily; a registered safety study (NCT07251608) uses the same 50 mg or 100 mg subcutaneous or intramuscular doses |
NAD+ dose to units chart
500 mg vial + 5 mL bacteriostatic water = 100 mg/mL (1,000 mcg per unit on a U-100 insulin syringe). 500 mg + 5 mL = 100 mg/mL, so 100 mg = 1 mL = 100 units (a full insulin syringe) and 50 mg = 50 units. Large doses mean large volumes; split a 100 mg shot across two sites if it stings.
| Dose | mL | Units (U-100) | Doses per vial |
|---|---|---|---|
| 750 mg | 7.5 | 750 | 0 |
| 1,000 mg | 10 | 1,000 | 0 |
Different vial or water? Full 500 mg + 5 mL table or set your own numbers below.
Side effects
- flushing
- chest tightness or pressure during fast delivery
- nausea
- injection site stinging
- headache
- cramping
NAD+ dosage questions
How much NAD+ to inject subcutaneously?
50 to 100 mg per shot, one to three times a week, is the commonly reported range and the range a registered safety study is testing. At 100 mg/mL that is 50 to 100 units. Start at 50 mg and inject slowly.
How much NAD+ is in an IV drip?
Clinics run 250 to 1,000 mg over 2 to 4 hours. The published human pilot used 750 mg over 6 hours at about 2 mg per minute, with no adverse events in 8 men. Faster rates are what cause chest pressure and nausea.
Can you take NAD+ every day?
Some people do 50 mg daily subcutaneously and some clinics do 5 IV sessions a week for 4 to 6 weeks. No trial has compared schedules. Most ongoing users settle at 1 to 2 shots a week.
Why does NAD+ injection burn or cause flushing?
NAD+ is a vasodilator and the solution is acidic at high concentration. Injecting slowly, using a lower concentration (more water) and splitting the dose between two sites cuts both the sting and the flush.
Sources
- Grant R et al., Front Aging Neurosci 2019, A pilot study of plasma and urine NAD+ metabolome during a 6 hour intravenous infusion of NAD+ (750 mg)
- ClinicalTrials.gov NCT07251608, safety study of subcutaneous and intramuscular NAD+ injections (50 mg or 100 mg)
- Peptide Protocol Wiki, NAD+ 100 mg dosing calculator (community subcutaneous ranges)
Brands we recommend for NAD+. We are independent and have no affiliation with any brand: no affiliate links, no referral codes, no paid placements. These are the suppliers whose testing and certificates hold up when we check them.
- Licensed Peptides two accredited labs on every lot, certificates public, card and Apple Pay checkout
- Core Peptides long track record, public certificates, wide catalog
- Ascension Peptides public third-party testing, clear pricing
This page is an educational summary of published labels, trials and commonly reported use. It is not medical advice and does not replace a clinician.