NAD+ calculator
NAD+ is a coenzyme, not a peptide, sold in 100 to 1,000 mg vials. Clinics run it IV at 250 to 1,000 mg over several hours; the one published human infusion study used 750 mg over 6 hours.
- Frequency
- subcutaneous: 1 to 3 times a week, up to daily; IV: 1 to 5 sessions a week in a course
- Timing
- morning or midday; it can be stimulating
- Cycle
- clinic courses run 4 to 6 weeks; subcutaneous use is often ongoing at 1 to 2 shots a week
- Route
- subcutaneous (abdomen) or IV in a clinic; inject subcutaneous doses slowly over a minute or more
- Easy mix
- 500 mg + 5 mL = 100 mg/mL, so 100 mg = 1 mL = 100 units (a full insulin syringe) and 50 mg = 50 units. Large doses mean large volumes; split a 100 mg shot across two sites if it stings.
NAD+ units chart
500 mg vial. Rows are doses; columns show the units to draw on a U-100 syringe at each water volume.
| Dose | mL | U-100 units | Doses per vial |
|---|---|---|---|
| 100 mcg | 0.001 | 0.1 | 5000 |
| 200 mcg | 0.002 | 0.2 | 2500 |
| 250 mcg | 0.0025 | 0.25 | 2000 |
| 500 mcg | 0.005 | 0.5 | 1000 |
| 1 mg | 0.01 | 1 | 500 |
| 2 mg | 0.02 | 2 | 250 |
| 2.5 mg | 0.025 | 2.5 | 200 |
| 5 mg | 0.05 | 5 | 100 |
| 10 mg | 0.1 | 10 | 50 |
Other mixes: 500 mg + 1 mL · 500 mg + 2 mL · 500 mg + 3 mL · 100 mg + 2 mL · 1000 mg + 2 mL
About the NAD+ math
NAD+, nicotinamide adenine dinucleotide, is a coenzyme found in every living cell. It is not a peptide, but peptide suppliers list it beside peptides, and the reconstitution math is identical: mass divided by volume gives concentration. The difference is scale. Vials are listed at 100 mg, 500 mg and 1,000 mg, so the arithmetic stays in milligrams and the solvent volumes are larger. A 100 mg vial with 2 mL of bacteriostatic water is 50 mg/mL. A 50 mg draw is 1 mL, or 100 units on a U-100 syringe, and the vial holds two. A 500 mg vial with 5 mL is 100 mg/mL, so 50 mg is 0.5 mL, or 50 units, with ten draws in the vial. A 1,000 mg vial with 10 mL is the same 100 mg/mL and holds twenty 50 mg draws; with 5 mL it is 200 mg/mL and 50 mg is 0.25 mL, or 25 units. Large masses need vials that can hold 5 mL or 10 mL, so check the vial capacity before choosing a volume.
NAD+ questions
How much NAD+ to inject subcutaneously?
50 to 100 mg per shot, one to three times a week, is the commonly reported range and the range a registered safety study is testing. At 100 mg/mL that is 50 to 100 units. Start at 50 mg and inject slowly.
How much NAD+ is in an IV drip?
Clinics run 250 to 1,000 mg over 2 to 4 hours. The published human pilot used 750 mg over 6 hours at about 2 mg per minute, with no adverse events in 8 men. Faster rates are what cause chest pressure and nausea.
How many units is 50 mg of NAD+ at 500 mg in 5 mL?
500 mg divided by 5 mL is 100 mg/mL. 50 mg divided by 100 mg/mL is 0.5 mL, which is 50 units on a U-100 syringe. The vial holds 10 such draws.
How much bacteriostatic water for a 1,000 mg vial of NAD+?
Any volume the vial holds. 1 mL gives 1,000 mg/mL, 2 mL gives 500 mg/mL and 3 mL gives 333 mg/mL (rounded). A 100 mg draw is 10, 20 or 30 units at those concentrations; larger volumes such as 10 mL give 100 mg/mL and a 100 mg draw of 100 units.
How many 25 mg draws are in a 100 mg vial of NAD+?
100 divided by 25 is 4 draws. With 2 mL of solvent (50 mg/mL) each 25 mg draw is 0.5 mL, or 50 units; with 1 mL (100 mg/mL) it is 0.25 mL, or 25 units.
Brands we recommend for NAD+. We are independent and have no affiliation with any brand: no affiliate links, no referral codes, no paid placements. These are the suppliers whose testing and certificates hold up when we check them.
- Licensed Peptides two accredited labs on every lot, certificates public, card and Apple Pay checkout
- Core Peptides long track record, public certificates, wide catalog
- Ascension Peptides public third-party testing, clear pricing